Establishment of a human malignant meningioma cell line with amplifiedc-myc oncogene

Object There is currently no effective chemotherapy for meningiomas. Although most meningiomas are treated surgically, atypical or malignant meningiomas and surgically inaccessible meningiomas may not be removed completely. The authors have investigated the effects of the topoisomerase I inhibitor irinotecan (CPT-11) on primary meningioma cultures and a malignant meningioma cell line in vitro and in vivo. Methods The effects of irinotecan on cellular proliferation in primary meningioma cultures and the IOMM-Lee malignant meningioma cell line were measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay and flow cytometry. Apoptosis following drug treatment was evaluated by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling and the DNA laddering assays. The effects of irinotecan in vivo on a meningioma model were determined with a subcutaneous murine tumor model using the IOMM-Lee cell line. Irinotecan induced a dose-dependent antiproliferative effect with subsequent apoptosis in the primary meningioma cultures (at doses up to 100 μM) as well as in the IOMM-Lee human malignant meningioma cell line (at doses up to 20 μM) irinotecan. In the animal model, irinotecan treatment led to a statistically significant decrease in tumor growth that was accompanied by a decrease in Bcl-2 and survivin levels and an increase in apoptotic cell death. Conclusions Irinotecan demonstrated growth-inhibitory effects in meningiomas both in vitro and in vivo. Irinotecan was much more effective against the malignant meningioma cell line than against primary meningioma cultures. Therefore, this drug may have an important therapeutic role in the treatment of atypical or malignant meningiomas and should be evaluated further for this purpose.

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EXTH-63. EFFICIENT ADCC-MEDIATED KILLING OF MALIGNANT MENINGIOMA CELLS USING AVELUMAB AND AN ENGINEERED HIGH AVIDITY NATURAL KILLER CELL LINE, haNK

Neuro-Oncology ◽

10.1093/neuonc/noy148.410 ◽

2018 ◽

Vol 20 (suppl_6) ◽

pp. vi98-vi98

Author(s):

Amber Giles ◽

Shuyu Hao ◽

Rika Fuji ◽

Michelle Padget ◽

Hua Song ◽

...

Keyword(s):

Natural Killer Cell ◽

Natural Killer ◽

Cell Line ◽

Killer Cell ◽

High Avidity ◽

Malignant Meningioma ◽

Natural Killer Cell Line

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14. Expression of myc oncogene prevents muscle cell fusion, but not biochemical differentiation or commitment in the C2C12 myogenic cell line

Biomedicine & Pharmacotherapy ◽

10.1016/0753-3322(92)90099-s ◽

1992 ◽

Vol 46 (5-7) ◽

pp. 245

Author(s):

M Crescenz ◽

F Tato

Keyword(s):

Cell Line ◽

Cell Fusion ◽

Muscle Cell ◽

Myogenic Cell ◽

Myc Oncogene ◽

Biochemical Differentiation

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Anti-Tumor Effect of a Novel DOX/GA-CdTe QD was Mediated by Apoptotic and Autophagic Cell Death

NANO ◽

10.1142/s1793292017500114 ◽

2017 ◽

Vol 12 (01) ◽

pp. 1750011 ◽

Cited By ~ 1

Author(s):

Huaqin Zuo ◽

Fan Wang ◽

Di Zhou ◽

Yi Zhou ◽

Bing Chen ◽

...

Keyword(s):

Cell Line ◽

Raji Cell ◽

Combined Treatment ◽

Burkitt’S Lymphoma ◽

Burkitt's Lymphoma ◽

Gambogic Acid ◽

Myc Oncogene ◽

Cdte Qds ◽

M Phase ◽

Relapsed Disease

Burkitt’s lymphoma is a highly proliferative B-cell malignancy characterized by MYC oncogene translocation. Intensive short-cycle chemotherapy could effectively improve the outcome of this disease. However, drug resistance limits the treatment of refractory/relapsed disease. Thus, we constructed and investigated a novel cadmium–tellurium quantum dot conjugated with doxorubicin and gambogic acid (DOX/GA-CdTe QDs) for cancer cell combined treatment in Raji, a Burkitt’s lymphoma cell line. Results showed that DOX/GA-CdTe QDs could significantly improve anti-tumor effects compared with drugs alone in the Raji cell line. Flow cytometry, transmission electron micrographs and overexpression of Beclin1 and LC3 II/I showed that apoptosis and autophagy were involved in the process. However, DOX/GA-CdTe QDs did not cause cell cycle arrest, whereas DOX alone or combined with GA could cause apparent G2/M phase arrest. Hence, the novel DOX/GA-CdTe QDs offer a promising approach of drug delivery into cancer cells.

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